Evidence against the Folate-mediated Formylation of Formyl-accepting Methionyl Transfer Ribonucleic Acid in Streptococcus faecalis R
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منابع مشابه
Evidence against the folate-mediated formylation of formyl-accepting methionyl transfer ribonucleic acid in Streptococcus faecalis R.
The formylation of formyl-accepting methionyl transfer ribonucleic acid (tRNA fMet) was investigated in Sfreptococcus faecalis R, an organism incapable of synthesizing folic acid but whose growth does not require the vitamin under deiined conditions of culture. These studies were carried out because it was not clear how an organism with no folate could initiate protein biosynthesis. Growth of S...
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The initiation of protein synthesis by Streptococcus faecalis R grown in folate-free culture occurs without N-formylation or N-acylation of methionyl-tRNA(f) (Met). Methionyl-tRNA synthetase and methionyl-tRNA formyltransferase were partially purified from S. faecalis grown under normal culture conditions in the presence of folate (plus-folate); the general properties of the enzymes were determ...
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The mechanism of the recognition of methionine by Escherichia coli methionyl-tRNA synthetase was examined by a kinetic study of the recognition of methionine analogues in the ATP-PPi exchange reaction and the tRNA-aminoacylation reaction. The results show that the recognition mechanism consists of three parts: (1) the recognition of the size, shape and chemical nature of the amino acid side cha...
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The MO-formyltetrahydrofolate:methionyl soluble ribonucleic acid transformylase has been purified over 1500-fold from Escherichia coli B. Although not homogeneous at this stage, the enzyme was of sufficient purity to determine some physical characteristics. The sedimentation constant was 2.02 which would indicate a molecular weight of approximately 25,000. In addition to the substrates of the r...
متن کاملThe postnatal methylation of transfer ribonucleic acid in brain. Evidence for the methylation of precursor transfer ribonucleic acid.
Incubation of 3-day-old rat brain with L-[methyl-3H]methionine resulted in the rapid labeling of low-molecular-weight cytoplasmic RNA. Electrophoresis in 15% polyacrylamide gels provided evidence for the methylation of precursor tRNA molecules, and high-performance liquid chromatography demonstrated N2-methylguanine to be the predominant methylated base formed during the first 2 min of labelling.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62825-x